Gas and Liquid Chromatography and Troubleshooting

Courses Syllabus

Introduction

Gas & Liquid Chromatography and Troubleshooting is a comprehensive course covering the following areas:

• Sample preparation
• Sample introduction
• Sample separation
• Columns
• Detectors
• Data acquisition
• Troubleshooting
• Reporting

Objectives

This program is designed to give the participants the capability to:

• Gain the basic theoretical aspects of Gas Chromatography
• Communicate practical information, capabilities and limitations of Gas & Liquid Chromatography
• Gain confidence on the analysis technique, troubleshooting and analytical results evaluation.

The Content

Introduction to Chromatography

• The History of Chromatography – GC & LC
• Overview of GC & LC Chromatography
• The Modern Chromatograph
• Liquid Chromatography – The Development Process
• Factors Controlling Retention
• Molecular Forces and Chromatographic Selectivity
• Effects of Stationary Phase Loading on the Performance of a Chromatographic System
• Chromatography Nomenclature

Chromatography Basics

• Basic Chromatography
• Sample Introduction
• The role of sample introduction and injection ports in GC operations
• Injection ports maintenance and its impact on GC performance
• Columns
• The role of columns in GC operations.
• Column selection & maintenance
• How columns can impact GC performance

GC Operation

• Peak Dispersion in a Chromatographic Column
• Detector Selection
• The role of Detectors in GC operations
• Detector maintenance
• How detectors can impact GC performance
• Setup and GC. Operation, Basic steps
• Preparation for operation.
• Sampling Techniques

Data Acquisition and Calibration

• Calibration
• Data Acquisition and Processing System
• Calibration linked to GC performance

Applications and troubleshooting

• Chromatography Applications
• Method Development
• Gas Chromatography
• Liquid Chromatography
• ISO17025 Accreditation Basics
• Laboratory Management & Troubleshooting